Activity: Alien DNA
Scenario: Alien DNA
NASA’s ‘Exobiology Branch’ (http://exobiology.nasa.gov/) supports research to increase knowledge on the origin, evolution, and distribution of life in the universe. An area of current research seeks to understand what life might look like beyond Earth. It is possible that the biochemistry and biological processes of extraterrestrial life are quite different than those on Earth. One of the areas of interest to researchers in this field is the genetic information system that extraterrestrial life may have evolved. Imagine that you are a member of a research team focusing on genetic information systems in samples collected from Mars by a probe that has just returned to Earth.
Initial analysis reveals the presence of eukaryotic, unicellular life in a sample. Additional analysis indicates the presence of double-stranded nucleic acids similar, although not identical, to DNA found in eukaryotic organisms on Earth. The alien nucleic acids consist of two strands of nucleotides bonded together, with the sugar-phosphates on the outside of the structure. Six nitrogen bases located on the interior of the double-stranded nucleic acid are identified as: isoAdenine (iA), Uracil (U), isoCytosine (iC), isoThymine (iT), Adenine (A) and betaGuanine (bG). Data also suggests a complementary base-pairing between nitrogen bases of the two strands with the following base paring rules:
For items 1-3 utilize the following information:
A double-stranded piece of alien nucleic acid has been isolated. Each individual strand of the nucleic acid is 160 nucleotides long; for a total of 320 nucleotides in the isolated piece of nucleic acid. An initial analysis shows that of the 320 nucleotides, 95 contain the base uracil.
With great anticipation, your team conducts tests on the alien nucleic acids. Media outlets from around the world are interested in reporting the results of your team’s research. The most fundamental questions to be answered include: Is the structure of the material similar to genetic systems of Earth? What is the mechanism of replication?
Initial analysis finds that the alien genetic material is a double-helix composed of nucleotides, with sugar-phosphates on the outside of the molecule and nitrogenous bases on the interior. The bases in the nucleotides of the alien genetic material are similar, but not identical, to those found in genetic systems on Earth. The alien nitrogenous bases are: isoThymine (iT), betaGuanine (bG), Adenine (A), isoAdenine (iA), uracil (U) and isoCytosine (iC). Studies reveal that the process of replication results in exact copies of the original nucleic acid. Below are results of tests to elucidate the nature of the alien replication process.
Below is a section of original alien genetic material showing the sequence of nucleotides in its two strands.
5’ 5’
iT – A
U – iA
A – iT
iA – U
iC – bG
U – iA
bG – iC
iT – A
iC – bG
3’ 3’
Below are the two molecules that are produced by replication of the alien genetic material above (*Note: ‘Cursive Font’ represents new nucleotides, ‘Print Font’ represents original/parental nucleotides).
5’ 5’ 5’ 5’
iT – A iT – A
U – iA U – iA
A – iT A – iT
iA – U iA – U
iC – bG iC – bG
U – iA U – iA
bG – iC bG – iC
iT – A iT – A
iC – bG iC – bG
3’ 3’ 3’ 3’
* Note: A / A = Adenine
U / U = Uracil
bG / bG = betaGuanine
iC / iC = isoCytosine
iT / iT = isoThymine
iA / iA = isoAdenine
Analyze the alien genetic material and answer the questions regarding its structure and method of replication.
For items 1-4, utilize the following information:
A piece of human double-stranded DNA is isolated. The piece of isolated DNA contains 410 nucleotides and 88 of those nucleotides contain the base adenine.
Note: ATGC = parental DNA
ATGC = new DNA
5′ 3′
T – A
G – C
C – G
A – T
G – C
A – T
3′ 5′
T – A T – A
G – C G – C
C – G C – G
A – T A – T
G – C G – C
A – T A – T
3′ 5′ 3′ 5′
T – A T – A
G – C G – C
C – G C – G
A – T A – T
G – C G – C
A – T A – T
3′ 5′ 3′ 5′
T – A T – A
G – C G – C
C – G C – G
A – T A – T
G – C G – C
A – T A – T
3′ 3′ 5′ 5′
T – A T – A
G – C G – C
C – G C – G
A – T A – T
G – C G – C
A – T A – T
3′ 5′ 3′ 5′