Determine the apparent vm and km values for new stat- Biology
An existing drug, OLD-STAT, is a competitive inhibitor of an enzyme that is too active in diabetes. The enzyme assay used to test and compare drug candidates is carried out at (S) = 0.15 mM. The enzyme has molecular weight of 28000, a turnover number of 13.8/sec, and the enzyme concentration is 6.49 mg/liter. The Km of the enzyme is 0.12 mM, and the inhibitor dissociation constant for OLD-STAT with the enzyme is 1.5 x 10-7 M.
A scientist in your company has identified a new drug candidate (NEW-STAT) that results in the enzyme reaction data shown in the plot below at a drug candidate concentration of 100 nanomolar.
a) Determine the apparent Vm and Km values for NEW-STAT
b)What kind of inhibitor is the new drug candidate? Explain.
c)Create plots of reaction rate (y-axis, V in mol/L/s) vs. substrate concentration (x-axis, C in mol/L) for:
i.the enzyme with no inhibitor,
ii.the enzyme with 100nM of OLD-STAT
iii.the enzyme with 100nM of NEW-STAT
d)Is NEW-STAT more or less effective in inhibiting the enzyme concentration at S = 1.5 mM?
