write the introduction regarding what you understand from the outline. And here are some points to add.
Introduction points:
What is male infertility
Causes
Diagnoses
Treatment: (ICSI, IVF)
In IVF many semen preparation techniques have been developed such as:
Density gradient techniques. (explain)
swim up
Semen analysis? sperm parameters and state the definitions of abnormal sperms and motility regarding WHO for example what is asthenozoospermia
little bit background of What is the principle of Nuclear magnetic resonance (NMR)? What is it used for?
AIMS and hypothesis
Overall aims:
Investigate why we see more metabolites in poor sperm than good sperm.
Examine how much seminal fluid contamination is in the two fractions using proInsert tubes vs Normal falcon tubes.
Hypothesis:
There are indications of seminal fluid contamination in poor sperm but not in good sperm.
Compare characteristic between 80% (good sperm ) and 40-80% interface (poor sperm) looking specifically on motility, morphology, ATP production and vitality.
Hypothesis:
Good sperm have a higher mean sperm speed, more progressive motility, more normal sperm morphology, a higher ATP production and few DNA fragmentations more than poor sperm.
To compare the above parameters between normozoospermic samples to asthenozospermic samples.
Hypothesis:
