Eukaryotic in vitro rna processing system/ Biology
Question: You are studying the promoter of the rpoA gene in E.coli, which has a -35 consensus sequence of TTGACA. If you mutate the -35 sequence to TTCAGA, will this have any effect on the transcription of the rpoA gene? Why or why not?
Question: You create a prokaryotic in vitro transcription system, focusing ot the trp gene in E. coli. To this system, you add all required components for successful transcription in E. coli, plus a compound that adds a third hydrogen bond between all base-paired RNA nucleotides. Will the trp gene be transcribed correctly? Why or why not?
Question: If you remove the methyltransferaseenzymes that can add methyl groups to nitrogenous bases, what will happen to mRNA processing in eukaryotes? Why?
Question: Construct a eukaryotic in vitro RNA procesing system, focusing on a 23S rRNA in the protist Tetrahymena. To this syste, you add all required components for successful RNA processing in Tetrahymena, except for one of the splieceosome snRNPs. Will the 26S rRNA be processed correctly? Why or why not? Be specific.
