Lab
Create a 2- to 3-page document in Microsoft Word for providing answers to questions in the following:
Exercise4: Purebacterialcolonies
1. Whenanagarplateis inoculated,whyis theloopsterilizedaftertheinitialinoculumis puton?
2. Distinguishbetweena purecultureandamixedculture.
3. Definea bacterialcolony.Listfourcharacteristicsbywhichbacterialcoloniesmaybe distinguished.
4. Whyshoulda Petridishnotbe leftopenforanyextendedperiod?
5. Whydoesthestreakingmethod youusedto inoculateyourplatesresultin isolatedcolonies?
Exercise5: Pourplateandstreakingtechniquetoobtainpurecultures
1. Discusstherelativeconvenienceofpour-andstreak-platetechniquesinculturingclinical specimens.
2. Howdoyoudecidewhichcoloniesshouldbepickedfrom aplatecultureofamixedflora?
3. Whyisitnecessarytomakepuresubculturesoforganismsgrownfrom clinicalspecimens?
4. Whatkindsofclinicalspecimensmayyielda mixedflorainbacterialcultures?
5. Whenmorethanonecolonytypeappearsinpureculture,whatarethemostlikelysourcesof extraneouscontamination?
Exercise3: Primarymediaforisolationofmicroorganisms
1.Definea differentialmediumanddiscussitspurpose.
2.Definea selectivemediumanddescribeitsuses.
3.WhyisMacConkeyagarselectiveas wellasdifferential?
4.Whyisbloodagarusefulasaprimaryisolationmedium?
5.Whatis themajordifferencebetweenModifiedThayer-Martin(MTM)andchocolateagar?Whenwould youuseMTMratherthanchocolateagar?
Support your responses with examples.
Cite any sources in APA format
