Prepare modeling recombinant DNA -Human insulin gene-Biology

Prepare modeling recombinant DNA -Human insulin gene-Biology

Answer the following as thoroughly as you can within this is a WORD document.Increase the space between questions as much as you need.

1. What are plasmids? Where are they found? Why are they important to the practice of genetic engineering?

2. Do plasmids have an importance beyond the practice of genetic engineering? Explain.

3. What are restriction enzymes?

4. What is the “origin of replication” on the plasmid and why is it important to the genetic engineering process?

5. What is the purpose of theantibiotic resistance genes(genes that codes for resistance to specific antibiotics) in the plasmid? Hint: The antibiotic resistance genes are used for screening purposes. Explain how they would aid in this process.

6. Why would you want your restriction enzyme to cut as close as possible to the insulin gene without cutting into it?

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