For discussion, discuss the purity in-depth. For protein is pretty sure, but not entirely pure. Why might that be? Also discuss why your protein eluted mostly in the 2nd and 3rd fractions, rather than the 1st fraction. Then, of course, a lengthy discussion of why the Western blot might not have worked, but what you should have seen which you have a good start on, and answering the questions on page 62.”
