Transformation efficiency is defined as the number of transformants per mg of DNA. pUC19 DNA (10 mL of a 0.005 mg/ml solution is added to 40 mL of E. coli cells in suspension. The solution is put into an electroporation cuvet and shocked. 450 mL of SOC media is quickly added and the cells are incubated at 37o for 60 minutes. Then, 10 mL of this solution is pipetted onto an LB/AMP plate and spread out. Following an overnight incubation at 37o, 576 transformed colonies were found growing on the plate.
a. How much DNA was in the electroporation cuvet?
b. How much DNA was in the 10 mL aliquot that was plated?
c. What is the transformation efficiency of this experiment
